制作缺血性中风气虚血瘀证大鼠模型的实验研究

Establishment of Rat Model of Ischemic Stroke with Qi Deficiency and Blood Stasis Syndrome and Its Evaluation System

目的探索建立缺血性中风气虚血瘀证大鼠模型的制作方法和评价体系。方法通过持续力竭性游泳复合线栓大脑中动脉闭塞法建立缺血性中风气虚血瘀证大鼠模型。观察模型鼠在表征、舌象、血液流变学及脑组织形态学等方面的指标变化,建立该模型的评价体系。结果持续力竭性游泳过程中模型鼠表现出体重增长相对缓慢,进食相对延缓,舌质变黯、舌下络脉增长等气虚血瘀状态的表征,线栓法手术后模型鼠表现出少动或不动,无力抵抗,偏瘫,舌色紫黯、舌下络脉青紫粗长等脑缺血气虚血瘀证的表征,血液流变学检测提示模型鼠血液黏度增高、血小板聚集性增高,脑组织形态学符合脑缺血改变特征。结论持续力竭性游泳复合线栓法可制备缺血性中风气虚血瘀证大鼠模型,表征、舌象、血液流变学及脑组织形态学等几方面指标的综合评价可构成该模型的评价体系。

Objective To probe into the establishing method and the evaluating system for rat model of ischemic stroke with qi deficiency and blood stasis syndrome （QDBS）. Methods A rat model of ischemic stroke of QDBS was established by continual exhaustive swimming followed with middle cerebral artery occlusion （MCAO）, and evaluated by analyzing the changes of exterior signs, tongue figure, hemorrheologic characters and brain histomorphology in the model rats. Results The model rats showed a state of QDBS in the course of continual exhaustive swimming, such as slower weight gain, postponed food intake, darker tongue and longer sublingual veins; and presented the characteristics of cerebral ischemia with QDBS syndrome after MCAO, they were inactive, weak, and hemiplegic, with dark purple tongue and longer blue sublingual veins. Moreover, hem- orrheologic examinations showed blood hyperviscosity and high platelet aggregation rate, and histomorphologic examinations showed a special figure of ischemic changes. Conclusion Continual exhausting swimming followed by MCAO is a method for establishing a rat model of ischemic stroke with QDBS syndrome, and its evaluating system could be constituted by multiple criteria, including exterior signs, tongue figure, hemorrheologic and histomorphologic indexes, etc.