染石英及石棉尘大鼠肺灌洗液SDS-PAGE测定

Analysis of SDSPAGE on BALF in Rats Exposed to Quartz or Chrysotile

目的探讨尘肺发病机理。方法采用ＳＤＳ－聚丙烯酰胺凝胶电泳（ＳＤＳ－ＰＡＧＥ）技术对染石英（２５ｍｇ／只）或石棉尘（５ｍｇ／只）大鼠支气管肺泡灌洗液（ＢＡＬＦ）进行了测定，并在光镜下观察天狼星红染色肺组织Ⅰ、Ⅲ型胶原分布情况。结果染石英不同时间大鼠ＢＡＬＦ中存在５条分子量分别为９０，６６，６０，２５，１４ＫＤ的蛋白质条带。染石棉大鼠ＢＡＬＦ仅有一条带，分子量为６６ＫＤ。染石英两周时，大鼠肺组织细胞纤维性结节中即有少量Ⅰ型胶原沉积，２个月时Ⅰ型胶原明显增加、粗大。石棉组３个月时肺间质中有Ⅰ型胶原存在。两组均未见明显Ⅲ型胶原。

n order to explore the pathogenesis of pneumoconiosis,SDSpolyacrylamide gel electrophoresis(SDSPAGE)was used to analyse the changes of bronchoalveolar lavage fluid(BALF)in male rats,at 05,1,2,3,5 months after intratracheal injection of quartz(25mg/rat)or Canada UICC chrysotile(5 mg/rat),and the lung histopathology with picrosirius stain were examined.The results showed that there were five protein bands in BALF of quartz exposed rats and their molecular weights were 90,66,60,25,14 KD,respectively;only oneband with 66KD molecular weight were found in BALF of chrysotile exposed rats.The collagens I deposited in pulmonary fibrotic nodes in quartz exposed rats could be seen in two week exposure,then gradually increased.Collagens I were only found in thickened lung interstitial tissues of chrysotile exposed rats after 3 month exposure,no collagens Ⅲ were found in two groups.The conclusion seems that the existing of proteins with different molecular weight in BALF of quartz exposed rats might play a promoting role in the development of silicosis.