摘要
目的在体条件下观察β-淀粉样蛋白25-35和31-35片段对大鼠海马CA1区长持续长时程增强(L-LTP)的影响。方法在体海马CA1区L-LTP记录技术;侧脑室给药技术。结果25nmol浓度的Aβ31-35或Aβ25-35不影响基础突触传递(n=5,P>0.05);Aβ25-35对L-LTP的抑制具有一定的剂量依赖性效应:6.25nmol,12.5nmol和25nmolAβ25-35组和对照组相比,显著抑制了L-LTP的维持(在高频刺激后3h:均P<0.05);12.5nmolAβ31-35同Aβ25-35,抑制L-LTP的维持(高频刺激后3h,P<0.05)。结论Aβ25-35和Aβ31-35可显著抑制在体海马L-LTP的维持,从而损害突触可塑性。
Objective To detect the effect of Aβ-protein(25-35 and 31-35)on the maintenance of hippocampal late phase of long-term potentiation(L-LTP)in vivo.MethodsMultiple HFSs was used to record hippocampal L-LTP and Aβ was given by intracerebroventricular(i.c.v.)injection.Results25 nmol Aβ25-35 or 31-35 did not affect the basic synaptic transmission(n=5,P〉0.05).Aβ25-35 showed a dose-dependent suppression on L-LTP:compared with control group,6.25,12.5 and 25 nmol Aβ25-35 obviously suppressed the maintenance of L-LTP(post-HFSs 3 h,all P〈0.05).12.5 nmol Aβ31-35 also significantly suppressed L-LTP at 3 h post-HFSs compared to control group(P〈0.05),which was the same as 12.5 nmol Aβ25-35.ConclusionsBoth Aβ25-35 and 31-35 can strongly suppress the maintenance of L-LTP in vivo and thereby impair hippocampal synaptic plasticity.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第7期777-780,共4页
Chinese Journal of Gerontology
基金
国家自然科学基金(30740095)
山西省青年科学基金(2008021045-2)