慢性乙型肝炎肝组织FasL的免疫组化研究

Immunohistochemical study of Fas ligand in liver tissue of chronic hepatitits B virus infection

目的通过研究程序性细胞死亡相关分子ＦａｓＬ在肝组织的表达情况，探讨慢性乙肝发病机制。方法以免疫组化方法检测６８例慢性乙肝活检组织。结果ＦａｓＬ主要表达在浸润的单个核细胞浆、肝细胞浆，其表达程度随组织炎症坏死程度加重而增加。结论ＣＴＬ通过Ｆａｓ机制导致感染的肝细胞凋亡是慢性乙型肝炎发病及炎症活动的重要机制之一。ＦａｓＬ阳性肝细胞可能发挥细胞毒效应，淋巴细胞凋亡的“顺序”和“转式”作用机制有可能存在于慢性肝炎的肝细胞。

To evaluate the role of apoptosis on liver injury of chronic hepatitis B,we studied the expression of Fas ligand and HBsAg immunohistochemically.Sixty-eight liver biopsy samples from patients with type B chronie liver disease were immunostained for Fas ligand and thirty-two of which were immunostained for HBsAg.35/68(51.47%) cases showed FasL immunoreactivie in liver-infiltrating mononuclear cells,and more expression of FasL were found in cytoplasm of hepatocytes.Both were closely linked to the degree of inflammation in liver(P=0.008 and P=0.011,respectively).But the expression of FasL had no close relationship with that of HBsAg in liver tissue (P>0.05).These findings suggest that apoptosis caused by CTL through Fas-mediated pathway plays an important role in liver cell injury by hepatitis B virus infection.At the same time, FasL-positive hepatocytes may express cytotoxic activity and eliminate themselves by both fratricide and suicide;`Bystander effect' can be used to account for the difference expression between FasL and HBsAg.