摘要
目的通过透射电镜观察烟曲霉孢子及代谢产物对肺泡巨噬细胞(PAM)吞噬功能形态学的影响。方法以体外培养的Wistar大白鼠PAM为靶细胞,制备烟曲霉孢子悬液和烟曲霉渗析物,用烟曲霉孢子混悬液刺激PAM后,加入烟曲霉蛋白或烟曲霉渗析物,观察PAM在吞噬烟曲霉孢子过程中的形态学变化,并与枝霉毒素,烟曲霉素和烟曲霉酸相对照。结果PAM可吞噬烟曲霉孢子,并发生明显的形态学改变。烟曲霉渗析物可抑制PAM对烟曲霉孢子的吞噬,枝霉毒素可诱导PAM发生凋亡。烟曲霉素和烟曲霉酸对PAM吞噬烟曲霉孢子的活动无明显影响作用。结论枝霉毒素和烟曲霉渗析物对PAM有一定的细胞毒性,烟曲霉渗析物能够抑制PAM的吞噬功能,枝霉毒素可诱导其发生凋亡。
Objective Pulmonary Alveolar Macrophages (PAMs) from Wistar rats were served in vitro as target cells to observe the phagotosis of Aspergillus fumigatus (A. f) spores and its metabolic products by electromnicroscopy. Methods The morphological changes of phagotosis of PAMs were observed by electronmicroscopy after exposure to A. f spores, Aspergillus fumigatus diffusate(AtD) or A.f proteins, and compard to gliotoxin, Fumagillin and helvolic acid. Results The morphological changes of PAMs were shown signfificantly after exposure to A. f spores. AfD could inhibit the phagotosis of PAMs partly. Moreover, gliotoxin could induce the apotosis of PAMs. Fumagillin and helvolic acid couldn't change the phagotosis of PAMs stimulated by A.fspores. Conclusion The results suggested that gliotoxin and AfD have cytotoxicity on PAMs, AiD could inhibit the phagotosis of PAMs and gliotoxin could induce the apotosis of PAMs.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2009年第4期196-198,共3页
The Chinese Journal of Dermatovenereology
关键词
烟曲霉
肺泡巨噬细胞
枝霉毒素
烟曲霉素
烟曲霉酸
吞噬活性
电镜
Aspergillus fumigatus
PAM
Branchi mildew toxins
Smoke song
Smoke aspergillus acid
Phagotosis
Electronmicroscopo