摘要
目的探讨甘草次酸(GA)对黑素瘤细胞侵袭能力和明胶酶表达的影响。方法体外培养黑素瘤细胞系SK-MEL-1,用MTT法检测不同浓度GA对细胞增殖的影响;采用Transwell小室基质胶侵袭实验观察GA对SK-MEL-1细胞株侵袭能力的影响;应用半定量RT-PCR检测GA对明胶酶A和B mRNA表达的影响;明胶酶谱法检测GA对SK-MEL-1细胞明胶酶活性的影响。结果SK-MEL-1细胞增殖抑制率与GA浓度及用药后培养时间呈正相关。GA对SK-MEL-1细胞侵袭能力的抑制与浓度相关,各浓度处理组与对照组差异均有显著性意义(P<0.01);GA能抑制SK-MEL-1细胞明胶酶A和B mRNA表达,与浓度呈正相关,各处理组与对照组差异有显著性意义(P<0.05);GA能够抑制SK-MEL-1细胞明胶酶活性,随浓度增高抑制作用增强,与对照组相比,120,240μmol/L浓度组差异具有显著性意义(P<0.05)。结论GA能够抑制黑素瘤细胞的增殖与侵袭能力,可能与抑制明胶酶的表达有关。
Objective To study the effect of invasiveness and expression of gelatinase on melanoma cell treated by glycyrrhetinic acid（GA）. Method MTT assay was performed to examine the effects of glycyrrhetic acid on the proliferation of SK-MEL- 1 cells cultured in vitro . The invasiveness of cells were tested by cell matrigel invasion assay. The expression of mRNA of gelatinase A and gelatinase B in the SK-MEL-1 cells treated by GA were assayed by semi-quantitied RT-PCR. Gelatin zymography was used to detect the activity of gelatinase in tested cells. Result GA showed marked inhibition on proliferation of SK-MEI-1 cells in time-and dose-dependentmanner. There was statistically significant difference in the invasiveness between the control group and GA groups. GA could downregnlate the expression of mRNA of gelatinase A and gelatinase B in treated SK-MEI-1 cells. GA could inhibit the activity of gelatinase of the treated cells,There was statistically significant difference between the control group and 120, 240μmol/L GA groups. Conclusion GA can inhibit invasiveness and the activity of gelatinase of the treated SK-MEL-1 melanoma cells,potentially, through inhibition of the expression of gelatinase. This provides the molecular basis for treatment of melanoma patients with this compound.
出处
《中国皮肤性病学杂志》
CAS
北大核心
2009年第4期207-210,共4页
The Chinese Journal of Dermatovenereology
