缺血致新生大鼠脑室周围白质软化和远期神经行为变化

Further neurobehavioral changes of the neonatal rats with periventricular leukomalacia induced by ischemia

目的:建立新生大鼠脑白质损伤模型,探讨脑白质损伤后少突胶质细胞(OL)凋亡和远期神经行为的变化。方法:5日龄新生大鼠随机分为实验组和对照组,制作缺血性脑白质损伤动物模型;术后不同时间点观察生长发育,HE染色、免疫荧光标记观察脑组织病理形态改变、细胞变化,悬吊试验、旷场试验、拒俘反应测试神经行为学改变。结果:实验组生长发育明显慢于对照组,HE染色脑室周围白质疏松,侧脑室增大,髓鞘磷脂蛋白(MBP)免疫荧光标记实验组阳性细胞数较对照组明显减少(P<0.05),神经行为学检测实验组大鼠神经行为能力减退。结论:成功建立5日龄新生大鼠脑白质损伤模型,显示新生大鼠脑白质损伤是以脑白质少突胶质细胞损伤为主的病变。

Objective To explore apoptosis of oligodendrocyte and the neurobehavioral changes of neonatal rats with hypoxic ischemie brain damage. Methods The neonatal rats （n = 90 ）at 5 days of age were randomly divided into experimental group（ n =48） and control group（ n = 42 ）. Bilateral carotids were ligated in the experimental group. The development of the rats at different time was observed. All rats in two group were killed at 2,3,9,16 and 25days after operation. The slices of the brains undertaken HE staining and immunohistochemistry staining were applied to investigate the pathologic manifestation, variation of oligodendrocyte and expression of myelin basic protein （MBP）. Hanging test, open field test and resist arrest reaction were performed on the rats 29 days and 30 days. Results The development of the rats of experimental group was delayed compared with the control group. HE staining showed white matter of brain loose and lateral ventricular dilatation,the expression of MBP had obvious reduction （P 〈 0.05） in the experimental group. The outcomes of neurobehavioral tests in experimental group were greatly abnormal compared with control group. Conclusions The models of white matter of brain injury are successfully established. Neonatal rats with ischemic brain injury shows obvious oligodendrocyte damage in white matter of brain.