孔雀石绿的催化共振散射法测定痕量过氧化氢

Enzyme Catalytic Determination of Trace Hydrogen Peroxide Using Malachite Green as Resonance Scattering Spectral Probe

在pH4．8的HAc-NaAc缓冲液中，辣根过氧化物酶（HRP）催化H2O2氧化过量KI生成I3^-，I3^-与孔雀石绿（MG）结合形成缔合微粒，导致体系的共振散射强度急剧增强。过氧化氢分别在6．86-274．8μg／L和6．86-343．4μg／L与486、720nm处的共振散射光强度成良好线性关系，检出限（3σ）为0．569、0．496μg／L。据此，建立一种测定痕量过氧化氢的共振散射光谱新方法。该法简便、快速，灵敏度高，用于雨水中过氧化氢的测定，结果满意。

A new resonance scattering spectral method was described for the determination of trace H2O2, based on the catalytic effect of horseradish peroxidase on the reaction between hydrogen peroxide and excess KI to I3^- in pH4.8 HAc-NaAc buffer solution. I3^- reacted with malachite green to form association particles,which enhanced the RS intensity greatly. The H2O2 concentration in the range of 6.86-274.8 μg/L and 6.86-343.4 μg/L was proportional to the resonance scattering peak at 486 nm and 720 nm, with detection limit （3a） of 0. 569 μg /L and 0. 496 μg /L respectively. The method was simple,rapid, highly sensitive,and had been applied for the determination of H2O2 of rain water with satisfactory re-sults.