支气管哮喘患者外周血嗜酸粒细胞上前列腺素D2受体改变研究

Change of prostaglandin D2 receptor on eosinophils in peripheral blood in bronchial asthma

目的了解不同病期支气管哮喘（简称哮喘）患者外周血中嗜酸粒细胞（EOS）上前列腺素D2（PGD2）受体（DP1／CRTH2）结合容量改变情况。方法随机病例对照研究。分为正常对照组24例，平均年龄（5．8±2．7）岁，哮喘急性发作期组18例，平均年龄（4．7±3．8）岁和缓解期组12例，平均年龄（3．9±3．1）岁。用放射配体结合分析法测定外周血EOS上DP1／CRTH2结合容量。结果哮喘急性发作期组和缓解期组患者EOS数显著增加（×10^6／L）（急性发作期组：297±58；缓解期组：157±34和对照组95±26）（P〈0．01），EOS上CRTH2受体结合容量和总结合容量比正常对照组和缓解期组显著增加（fmol／10^6细胞）（CRTH2分别为：245．58±48．27、154．13±67．25、119．29±53．47；DP总结合分别为：456．14±25．57、337．51±38．52、301．22±39.54）（P〈0．01）。结论哮喘急性发作期组CRTH2受体结合容量增加，PGD2激发的EOS的趋化反应将偏向于CRTH2受体中介的生物学效应。

Objective To investigate the change of prostaglandin De receptor （DP1/CRTH2） on eosinophil（EOS） in exacerbation and remission phase patients with bronchial asthma（asthma）, Methods Randomized case control clinical trial was designed. Thirty patients were randomly divided into two groups： exacerbation phase group 18 cases（4.7±3.8） years, and remission phase group （3.9 ± 3.1 ） years, normal control was 24 cases（5.8±2.7） years. Radiological binding assay was used to study the DP1/CRTH2 receptors on EOS in peripheral blood. Results The EOS counts in exacerbation phase group were significantly higher than those in remission phase group and normal controls（ 〉（ 10^6/L） （exacerbation phase group,297±58, remission phase group 157 ± 34 normal controls 95± 26） （ P 〈0.01 ）. The combining capacity of CRTH2 and the total combining capacity on EOS in exacerbation phase group were significantly higher than those in remission phase group and normal controls（fmol/10^6 cell）（245.58±48.27 vs 154.13 ± 67.25,119.29±53.47 and 456.14±25.57,337.51±38.52,301.22±39.54）（ P〈0.01）.Conclusions The combining capacity of CRTH 2 on EOS in exacerbation phase group are significantly higher, this imply that PGD2 mediates the chemotaxis of EOS will be via CRTH2 receptor.