PPARγ、PTEN在乳腺导管上皮良、恶性病变中的表达及意义

The expression and significance of PPARγ and PTEN in intraductal proliferative lesions and invasive ductal carcinoma

目的:研究过氧化物酶增殖物激活受体γ(PPARγ)和10号染色体缺失且与张力蛋白同源的磷酸脂酶基因(PTEN)在乳腺导管上皮良、恶性病变中的表达及其在乳腺癌发生发展中的作用。方法:应用PV-9000二步法免疫组化进行染色,检测30例乳腺导管上皮普通性增生(UDH)、30例不典型增生(ADH)、30例导管原位癌(DCIS)及60例浸润性导管癌(IDC)石蜡包埋组织中PPARγ和PTEN的表达。结果:(1)PPARγ在UDH、ADH、DCIS及IDC中的阳性高表达率呈递增趋势,依次为16.7%(5/30)、43.3%(13/30)、50.0%(15/30)、60.0%(36/60);PTEN呈递减趋势,依次为73.3%(22/30)、63.3%(19/30)、40.0%(12/30)、35.0%(21/60)。(2)PPARγ在UDH组与ADH组、ADH与IDC组的表达差异有统计学意义(P<0.05);PTEN在ADH与DCIS组的表达差异有统计学意义(P<0.05)。(3)PPARγ和PTEN在乳腺浸润性导管癌中的阳性表达呈负相关(r=-0.3236,P<0.05)。结论:PPARγ、PTEN在乳腺导管上皮良、恶性病变中的异常表达与肿瘤的发生、发展密切相关,检测PPARγ、PTEN有可能成为预测乳腺癌发生、发展及预后的指标。

Objective： To study the expression of PPARγ and PTEN in intraductal proliferative lesions, invasive ductal carcinoma and analyze the influence of breast cancer. Methods： The expression of PPARγ and PTEN in 30 patients with usual ductal hyperplasia （UDH）, 30 patients with atypical ductal hyperplasia （ADH）, 30 patients with ductal carcinoma in situ （DCIS）, and 60 patients with invasive ductal carcinoma （IDC） were detected by PV-9000 immunohistological method. Results： （1） The positive rate of PPARγwas 16.7% in UDH, 43.3% in ADH, 50% in DCIS, 60% in IDC. The positive rate of PTEN was 73.3% in UDH, 63.3% in ADH, 40% in DCIS, 35% in IDC. From UDH, ADH, DCIS to IDC the expression of PPARγ showed an ascending tendency, but the expression of PTEN showed an descending tendency. （2） PPARγ high expressive rates in UDH and ADH were 16.7%（5/30） and 43.3%（13/30）, which was significantly different from that in IDC 60%（36/60） （P 〈0.05）. PTEN high expressive rate in ADH was significantly different from the expression in IDC （P 〈0.05）. （3） PTEN expression was negatively correlated to the expression of PPARTin invasive ductal carcinoma （r =-0. 323 6, P 〈0.05）. Conclusion： Abnormal expression of PPARγ and PTEN in intraductal proliferative lesions and invasive ductal carcinoma indicated that were relation of occurring and development, contributing to the invasion of breast cancer, it may be useful to foreeast development of by breast cancer examine.