罗勒提取物对肾性高血压大鼠的影响

Antihypertensive effects of Ocimum Basilicum L (OBL) on renal hypertensive rats

目的:通过验证罗勒(全草)提取物(OBL)对肾性高血压模型大鼠的降压作用,探讨OBL的降压作用机理。方法:正常Sprague-Dawley(SD)大鼠适应性喂养1周后测基础血压。然后进行左肾动脉狭窄手术。手术后4周,形成两肾一夹(2K1C)肾性高血压。将高血压大鼠60只随机分成5组:罗勒提取物高剂量组(H,400 mg/kg)、罗勒提取物中剂量组(M,200 mg/kg)、罗勒提取物低剂量组(L,100 mg/kg)、卡托普利组(Cap,30 mg/kg)、高血压模型组(Model)。从肾动脉狭窄手术后4周开始灌胃给予相应药物,持续4周,每周末测血压;外加假手术组(Sham)10只。处死动物取血测血管紧张素Ⅱ(AngⅡ)、内皮素(ET)、超氧化物歧化酶(SOD)、丙二醛(MDA)。结果:两肾一夹(2K1C)术后4周,假手术组血压为(138±2.04)mmHg,模型组血压为(201±3.69)mmHg,两组血压差异有统计学意义(P<0.05)。同时AngⅡ、ET以及MDA含量明显增高;而SOD活性降低。给予罗勒提取物后血压(收缩压和舒张压)均有下降。ET、MDA明显下降、而SOD活性增高(P<0.05),且作用强于卡托普利组(P<0.05)。同时AngⅡ也明显下降。结论:罗勒提取物对肾性高血压大鼠具有降压作用,其降压作用机制可能与通过抗氧化反应来保护内皮细胞,改善血管内皮功能紊乱,抑制缩血管物质(内皮素、血栓素A2、血管紧张素Ⅱ)的释放有关。

Objective： To investigate the effects of the extraction of Ocimum basilicum L （OBL） on renal hypertensive rats and investigate its mechanism. Methods： SD rats received food and water adlibiturn in 1 week of acclimation. After that baseline blood pressure （systolic and diastolic blood pressure） was measured by noninvasive tail cuff photosensor under aseptic conditions, thirty general anesthesia rats were given a modifided 2 kidney-1 clip （2K1C） operation while six rats were sham-operated （the animals received identical treatment without left renal artery ligation）. Four weeks later, hypertensive rats were randomly allocated to four groups such as OBL （higher, 400 mg/kg）, OBL（middle, 200 mg/kg）, OBL（lower, 100 mg/kg）, captopril （cap, 30 mg/kg） and model group after allocation, the treatment period was 4 weeks. The blood pressure was measured weekly. At sacrifice, blood was harvested to measure the levels of angiotensin Ⅱ fAng Ⅱ ）, superoxide dismutase （SOD）, malonaldehyde（MDA） and endothelin （ET） at the end of the 4th week. Kidneys and hearts were collected to observe the pathological changes of arteriole too. Results： Four weeks after 2KlC operation, the blood pressure of the 2kidney-lclip operation group increased significantly （201＋3.69） mmHg vs （138±2.04） mmHg of sham group （P 〈0.05）. And the levels of AngⅡ , MDA, ET was slightly increased in plasma. SOD was decreased significantly. Treated with captopril and OBL, the blood pressure （systolic blood pressure and diastolic blood pressure） decreased significantly and continually （P 〈0.05）. During the course of administration, the levels of Ang Ⅱ , MDA and ET were slightly decreased in plasma. Mean while, SOD in plasma increased respectively （P 〈0.05）. Conclusions： OBL can reduce the blood pressure of the renal hypertensive rat, Which is likely related to through the antioxidate reaction, to improve the blood vessel endothelium function. It should be more than one antihy-pertensive target of OBL.