抗旱植物沙冬青对植物激素反应特性的研究

The Effect of Phytohormone on Ammopiptanthus Mongolicus, a Drought Resistant Plant

以沙冬青为材料,选用茎尖、茎段、子叶和下胚轴为外植体进行组织培养,主要研究植物激素6-BA、KT在芽分化中的作用,结果表明:茎尖和茎段在含1mg/L和2mg/L 6-BA的培养基上,形成了玻璃化的丛芽而死亡,在含低浓度6-BA(0.5mg/L,0.1mg/L,0mg/L)的培养基上,能够产生一定比例、无玻璃化生长正常的芽,以6-BA含量为0.5 mg/L分化的芽率较高。在子叶和下胚轴的分化过程中,0.5 mg/L的6-BA,能够提高子叶不定芽的分化频率,作用较KT明显,相反0.5 mg/L KT提高了下胚轴不定芽的分化。

The effect of Phytohormone 6-BA and KT on Ammpiptanthus Mongolicus shoot differentiation during tissue culture had been studied with stem apex, stem segment, cotyledon, hypocotyl as explants respectively. Results showed that： shoots can regenerate from stem apex and stem segment when culture in MS medium with 1 and 2mg/L 6-BA, but the shoot leaf then vitrification and died. Normal shoot can formed when cuhured in low 6-BA medium （0.5,0.1,Omgh~）. The shoot differentiation frequency is better higher in 0.5mg/L 6-BA. 0.5mg/L 6-BA can increase the differentiation frequency of cotyledon explants, and 0.5mg/L KT can increase the shoot differentiation frequency of hypocotyt during the differentiation of cotyledon and hypocotyl.