氮和磷对红发夫酵母突变株sp12生长和虾青素合成的影响

Effects of Nitrogen and Phosphorus on Growth of the Mutant Strain Sp12 of Phaffia rhodozyma and Synthesis of Astaxanthin

采用无机培养基,以NaNO3、Na3PO4为氮、磷源培养红发夫酵母。结果表明,红发夫酵母生长对氮的需求量较低,当培养基中NaNO3含量低于10.20 mmol/L(870 mg/L)时,酵母生物量无显著变化,当NaNO3含量高于10.20 mmol/L时,其生物量略有下降;无磷严重抑制红发夫酵母生长,当培养基中Na3PO4含量低于1.22 mmol/L(200 mg/L)时,酵母生物量随Na3PO4含量的增加而增加,且Na3PO4含量为1.22 mmol/L时,酵母生物量最大,当Na3PO4含量高于1.22 mmol/L时,酵母生物量下降;氮、磷对虾青素的合成均有显著影响;当培养基中葡萄糖浓度为10 g/L时,氮、磷的最佳添加量分别为9.06、1.22 mmol/L(770和200 mg/L),即氮、磷最佳配比为7.5∶1.0(摩尔比)。

With NaNO3,Na3PO4 as nitrogen and phosphorus source,the inorganic medium was taken to culture phaffia rhodozyma.The results showed that the demand amount of growth of phaffia rhodozyma on nitrogen was low.When the NaNO3 content in medium was lower than 10.20 mmol/L（870 mg/L）,the yeast biomass had no significant change,and when the NaNO3 content in medium was higher than 10.20 mmol/L,the yeast biomass decreased a little.The growth of phaffia rhodozyma could be seriously inhibited when there were no phosphoru in medium. When the Na3PO4 content in medium was lower than 1.22 rranol/L（200 mg/L）, the yeast biomass increased with the increasing of Na3PO4 content, and the yeast biomass was the maximum when the Na3PO4 content was 1.22 mmol/L, the yeast biomass decreased when Na3PO4 content was higher than 1.22 mmol/L. Nitrogen and phosphorus both had significant effect on the synthesis of astaxanthin. When the glucose content in medium was 10 g/L, the optimum content of nitrogen and phosphorus were 9.06 and t .22 mmol/L（770 and 200 mg/L） , respectively, that is the optimum proportion of nitrogen and phosphorus was 7.5：1.0 （molar ratio）.