儿童过敏性哮喘尘螨特异性免疫治疗中干扰素-γ白介素4T-bet和GATA-3 mRNA表达变化的研究

Changes of expression of IFN-γ,IL-4,T-bet and GATA-3 mRNA during house dust mites specific immunotherapy for children with allergic asthma

目的探讨在过敏性哮喘尘螨特异性免疫治疗(SIT)过程中,与TH1/TH2细胞分化相关的干扰素-γ(IFN-γ)、白介素4(IL-4)、T-bet和GATA-3 mRNA在外周血单个核细胞(PBMCs)中表达的变化。方法根据进行抗原特异性免疫治疗(SIT)的时间,采集郑州大学第一附属医院儿科门诊2007年11月至2008年9月收治的过敏性哮喘患儿36例,对其SIT治疗前、治疗16周后和治疗1年后的外周静脉血分离外周血单个核细胞,提取总RNA,逆转录成cDNA,利用SYBR GreenⅠ荧光定量PCR的方法检测每组中IFN-γ、IL-4、T-bet和GATA-3 mRNA表达情况。同时,观察患儿治疗前后的临床疗效。结果随着SIT治疗的进行,患儿临床症状明显减轻,外周血中在mRNA表达水平IFN-γ/IL-4和T-bet/GATA-3的比值逐渐升高。结论哮喘患儿特异性免疫治疗有效;治疗后TH1/TH2细胞的失衡得到一定的纠正;T-bet和GATA-3转录因子在哮喘的发生和SIT治疗中起重要作用。

Objective To survey the changes of expression of IFN-γ, IL-4, T-bet and GATA-3 mRNA in peripheral blood mononuclear cells （PBMCs） in house dust mites specific immunotherapy （SIT） for patients with allergic asthma. Methods The periphery venous blood of the allergic asthma patients was harvested three times ： pre-SIT, post-SIT for 16 weeks and post-SIT for 1 year. The IFN-γ, IL-4, T-bet and GATA-3 mRNA in PBMCs were detected by SYBR Green I RT-PCR. At the same time, the clinical effects were detected. Results As the progression of the SIT, the clinical symptom was alleviated, and the ratios of IFN-γ to IL-4 and T-bet to GATA-3 on mRNA expression level rised gradually. Conclusion The SIT is efficient for the patients with allergic asthma; the disequilibrium of TH1/TH2 is retrieved; the transcription factors T-bet and GATA-3 may play an important role in pathogenesis of asthma and the SIT.