补益阳明津气对初老雌性大鼠卵巢Caspase3表达影响的实验研究

Explore the effect and mechanism of "tonifying Yang Ming Fluid and Qi" to expression of caspase3 of the primary senile rat ovary

目的：探讨“补益阳明津气”延缓初老雌性大鼠卵巢机能衰老的机理，并以此佐证中医传统经典理论“五七，阳明脉衰”在绝经前期生殖轴机能衰老中的重要影响。为促进绝经前期妇女健康，延缓绝经前期卵巢机能衰老，防治相关病症，提供-种新的研究与论治思路，并为研制相关方药提供药效学依据。方法：4～6个月龄雌性大鼠为正常对照组；10～12个月龄，I舛道细胞学表现动情期延长的雌性大鼠作为初老大鼠模型。模型动物随机分为：（1）益胃汤高剂量组；（2）益胃汤中剂量组；（3）益胃汤低剂量组；（4）己烯雌酚组；（5）模型对照组；（6）六味地黄丸组。灌药4周后，断头处死，取左侧卵巢用于检测卵巢Caspase3。结果：与模型对照组比较，使卵巢Caspase3表达减少。结论：实验研究提示益胃汤具有抑制卵巢细胞凋亡的作用，这可能是其延缓卵巢机能衰老的机理之一。

Objective：To explore the effect and mechanism of ＂tonifying Yang Ming Fluid and Qi＂ to delay the aging of the primary senile rat ovary and so that to testify the importance of TCM traditional classic theory ＂Yang Ming Meridian Declines during Wu Qi period＂ in premenopause. The study may offer a new study and differentiational thought to improve premenopausal women health, delay the aging of ovary founction, prevent and treat relative diseases. It also can offer pharmacodynamics evi- dence for manufacturing relative effective formulas. Methods ：4 ~ 6 months old SD female rats were set to be normal control group and 10 ~ 12 months old SD female rats whose oestrus elongation were set as primary senile female rat model. Primary senile female rats were devided into 6 groups randomly as following： （ 1 ） High dose Yiwei tang group; （2） Middle dose Yiwei tang group; （ 3 ） Low dose Yiwei tang group; （4） Diethylstilbestrol group; （5） Model control group; （6） Liuwei dihuang wan group. The intervention period was 4 weeks. The ovaries were treated by Immune histochemistry method to observe expression of Caspase3. Results ： Yiwei tang could decrease the expression of Caspase3. Condusion：The results showed that Yiwei tang, which could tonify Yang Ming Fluid and Qi, could restrain ovary apoptosis. The effect may be one of the mechanism of Yiwei tang delay the aging of primary senile rat ovary.