苦参碱对雄激素依赖性前列腺癌细胞的影响

Effect of Matrine on Androgen Dependent Prostate Cancer Cells

目的:探讨苦参碱(Matrine)对雄激素依赖性前列腺癌细胞株(LNCaP)凋亡及前列腺特异抗原(prostates pecific antigen,PSA)表达的影响。方法:分别用0.5 g/L、1.0 g/L、1.5 g/L、2.0 g/L浓度的苦参碱作用于LNCaP细胞12 h、24 h、36 h后MTT法检测细胞生长活性;24 h后流式细胞仪测定细胞凋亡的变化;24 h后Western印迹法检测细胞内Bcl-2和Bax的表达;12 h、24 h、36 h后化学发光法检测LNCaP细胞培养液中PSA的变化。结果:苦参碱能抑制LNCaP细胞的生长,呈剂量与时间依赖性,不同浓度苦参碱组之间与不同作用时间组之间的差异均有统计学意义(P<0.05)。苦参碱诱导LNCaP细胞凋亡,各浓度组凋亡细胞比例均显著高于对照组,差异有统计学意义(P<0.05);LNCaP细胞内Bcl-2含量呈浓度依赖性下降,Bax含量呈浓度依赖性升高(P<0.01);LNCaP细胞培养液中PSA的表达显著下降(P均<0.05)。结论:苦参碱能显著抑制LNCaP细胞的体外生长,诱导其凋亡,并抑制PSA的表达。

Objective：To study the effect of matrine on the apoptosis of androgen dependent prostate cancer cells LNCaP and the expression of prostate specific antigen （PSA）. Methods： After being treated with 0.5g/L, 1.0 g/L,1.5 g/L,2.0 g/L matrine for 12 h,24 h,36 h, the growth activities of LNCaP cells were studied by MTT colorimetry. The apoptosis were inspected by flowcy-tometery （FCM） after 24h. The Levels of Bcl-2 and Bax were tested by Western Blotting. The expression of PSA in LNCaP cells culture medium was measured by AX- SYM system-chemical luciferase methods. Results： Matrine could effectively inhibit the in vitro growth of androgen dependent prostate cancer cell line LNCaP in time-and-dose-dependent manners. Different concentrations of matrine group and the different of time groups were all have statistically significant （P〈0.05）. Partial cancer cells were presented apoptosis by matrine. The proportion of apoptotic cells in each concentrations of matrine group were significantly higher than the control group （P〈0.05）. Matrine could decrease in the levels of Bcl-2 and increase in the levels of Bax （P〈0.01）in cell line LNCaP in a dose-dependent manner. Matrine inhibited the expression of PSA on prostate cancer ceils （P〈0.05）. Conclusions： Matrine can suppress the growth of LNCaP cells, and promotes their apoptosis. Matrine also inhibited the expression of PSA on prostate cancer cells.