转移抑制基因Kiss-1在舌鳞癌原发灶和淋巴结转移灶的表达

Expression of metastasis suppressor gene Kiss-1 in tongue squamous cell carcinoma and their lymph node metastatic tumours

目的:探讨肿瘤转移抑制基因Kiss-1在舌鳞癌原发灶和淋巴结转移灶的表达。方法:采用免疫组织化学SP法检测59例舌鳞癌标本原发灶癌组织和其中21例发生淋巴结转移的转移灶中Kiss-1蛋白的表达,并分析其与临床病理参数的关系。结果:59例原发灶标本中Kiss-1蛋白的表达率:无淋巴结转移组为97.37%(37/38),有淋巴结转移组为90.47%(19/21),两组相比差异无统计学意义(p>0.05);但有淋巴结转移组的表达强度低于无淋巴结转移组,两组差异有统计学意义(p<0.05)。在21例发生淋巴结转移的病例中,淋巴结转移灶表达率为28.57%(6/21),明显低于原发灶的90.47%(19/21),两组差异有统计学意义(p<0.01)。Kiss-1蛋白表达与患者的年龄、性别、临床分期、组织学分级、嗜烟酒等因素无关(p>0.05)。结论:舌鳞癌中Kiss-1蛋白表达减弱或丢失可能是导致舌鳞癌转移能力增强的因素之一。

Objective： To study the expression of Kiss-1 in primary and lymph node metastasistic lesions in tongue squamous cell carcinoma （TSCC）. Method： Immunohistochemical SP staining was used to detect the expression of Kiss-1 protein in 59 primary tongue squamous cell carcinoma and their lymph node metastatic tumours （21 with lymphatic metastasis）.Result：①The positive rate of Kiss-1 were 90.47% in TSCC with lymph node metastasis and 97.37% in TSCC without metastasis,respectively （p〉0.05）. Expressive intensity in TSCC with lymph node metastasis was lower than that without metastasis （p〈0.05）.②The positive rate of Kiss-1 in metastatic lymph node site was significantly lower in primary site of TSCC （28.57%, 90.47%） （p〈0.01）.③There was no correlation between the expression of Kiss-1 and age, sex, clinical stage, histological grade, philo-smoke, philo-alcohol separately （p〉0.05）. Conclusion： The loss or decreasing of expression of Kiss-1 may be a main factor in metastasis of TSCC.