肝细胞癌组织中PPARα mRNA的表达规律及其意义

Expression of PPARα mRNA in Liver of Primary Hepatocellular Carcinomas

目的探讨过氧化物酶体增殖物激活受体α(peroxisome proliferator activated receptorα,PPARα)mRNA在肝细胞癌(HCC)中的表达规律及其临床意义。方法通过荧光定量PCR法测定22例患者HCC组织及相对应的癌旁组织PPARα mRNA表达变化。结果79.4%(17/22)HCC组织PPARα mRNA表达显著低于癌旁组织(P<0.05)。结论HCC组织中PPARα mRNA表达下调,这种变化可影响肝细胞的脂质代谢过程,与HCC的发展可能有关,可作为判断HCC预后的一种预测性指标。

Objective To investigate the expression of peroxisome proliferator activated receptor（PPARα） mRNA in liver of primary hepatoeellular carcinomas. Methods Total RNA of liver was isolated with trizol and the levels of liver PPARα were measured by reverse transcription polymerase chain reaction（RT-PCR） in HCC tissue and the other tissues near the tumor from 22 patients after surgical treatment. Results Compared with other tissues, PPARα mRNA levels in HCC tissues were significantly decreased in liver during 17/22.（P〈0.05）. Conclusion HCC tissues can decrease the expression of PPARα mRNA in liver, which might be affect the course of lipid metabolized of liver cell, which might be involved in the initiation and the development of HCC. So we can judge the prognosis by it.