人牙周膜细胞在透明质酸/胶原支架上的黏附与生长

Adhesion and growth of human periodontal ligament cells on hyaluronic acid/collagen scaffold

目的研究人牙周膜细胞(PDLC)在胶原、透明质酸及透明质酸/胶原支架上的黏附、生长情况,以初步探讨透明质酸/胶原支架应用于牙周组织工程的可行性。方法将体外培养的人牙周膜细胞接种到碳化二亚胺交联的胶原、透明质酸及透明质酸/胶原支架上;MTT法检测支架对人牙周膜细胞黏附、生长的影响;并用倒置相差显微镜和扫描电镜观察形态变化。结果MTT结果显示胶原、透明质酸及透明质酸/胶原支架上人PDLC的黏附、生长情况,在第1、2、4天组间比较差异具有统计学意义(P<0.05),第7天组间差异无统计学意义(P>0.05),且人牙周膜细胞数量透明质酸/胶原组均高于对照组;人牙周膜细胞在支架上生长良好。结论相对于胶原支架和透明质酸支架,透明质酸/胶原支架更有利于人牙周膜细胞的黏附,提示该材料具备成为牙周组织工程理想支架材料的潜力。

Objective The adhesion and growth of human periodontal ligament cells （PDLC） on collagen （Col）, hyaluronic acid （HA） and HA/Col scaffolds were studied to evaluate the feasibility of HA/Col as a scaffold material in periodontal tissue engineering. Methods Human PDLC cultured in vitro was collected and seeded on Col, HA, HA/Col scaffolds crosslinked by carbodiimide. The influences of scaffolds on cell adhesion and growth were observed by MTT assay. The growth of human PDLC on scaffolds was observed through inverted phase contrast microscope and scanning electron microscope （SEM）. Results The result of MTr assay demonstrated that except the seventh day there was obviously differentiation between each group Col, HA, HA/Col scaffolds at the first, second and fourth day, and HA/Col group was higher than control group all the time. Human PDLC successfully grew on scaffolds. Conclusion HA/Col scaffold was more suitable for human PDLC adhesion compared with Col scaffold and HA scaffold. This indicates that HA/Col scaffold might be a potential ideal candidate for periodontal tissue engineering.