促红细胞生成素促进大鼠坐骨神经再生作用的实验研究

Experimental study of erythropoietin on promoting sciatic nerve regeneration in rats

目的探讨促红细胞生成素（Erythoropoietin，EPO）对大鼠坐骨神经损伤修复后神经再生的影响，为周围神经损伤的临床治疗提供实验依据。方法雄性SD大鼠40只，随机分为两组，即EPO组和神经生长因子（NGF）组，用硅胶管桥接10mm的坐骨神经缺损，EPO组和NGF组分别注射EPO和NGF。术后4周和8周时每组分别提取10只大鼠，以坐骨神经功能指数（SFI）、运动神经传导速度（MNCV）、形态学观察和蛋白基因产物9．5（PGP 9．5）免疫组织化学染色，评估EPO对大鼠坐骨神经再生的影响。结果术后4周SFI，EPO组为[（-78．85±3．87），^-x±s，下同]，NGF组为（-79．98±4．58），差异无统计学意义（P〉0．05）；术后8周SFI，EPO组为（-60．26±2．91），NGF组为（-64．65±4．11），差异有统计学意义（P〈0．05）。术后4周和8周时，EPO组MNCV、有髓神经纤维数目以及PGP9．5免疫阳性神经纤维的平均光密度和积分光密度均优于NGF组，差异有统计学意义（P〈0．05）。结论EPO能促进大鼠坐骨神经损伤后的修复与再生。

Objective To explore the effect of erythropoietin （EPO） on nerve regeneration after sciatic nerve injury and repair in rats and provide experimental basis for clinical treatment of peripheral nerve injury. Methods Forty male Sprague-Dawley rats were randomly divided into two groups, which were named EPO group and NGF group respectively . The sciatic nerve with 10 mm defect was bridged with silicone conduit. Rats of EPO group and NGF group received injection of erythropoietin and nerve growth factor respectively. At 4 and 8 weeks postoperatively, 10 rats from each group were subject to sciatic functional index （SFI）, motor nerve conduction velocity （MNCV） and histomorphometric evaluation and immunohistochemical staining of protein gene product 9. 5 （PGP 9. 5） to assess the effect of erythropeietin on rat sciatic nerve regeneration. Results Four weeks after the operation , SFI was - 78.85 ± 3.87 in EPO group and - 79.98 ± 4.58 in NGF group. There was no significant difference between the two groups（ P 〉 0.05）. Eight weeks postoperatively , SFI was - 60.26 ± 2.91 in EPO group and - 64.65 ± 4.11 in NGF group, with significant difference between the two groups（ P 〈 0.05）. Four and eight weeks after the operation, MNCV, the number of myelinated nerve fibers, the mean optical density and the integration optical density of PGP 9.5-immunoreactive nerve fibers were significantly higher in EPO group than in NGF group （ P 〈 0. 05）. Conclusion Erythropoietin can improve nerve regeneration after sciatic nerve injury and repair in rats.