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稳定过表达p50基因细胞株的构建及鉴定

Construction and appreciation of p50 gene cells stable overexpression
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摘要 目的筛选出稳定过表达p50基因的细胞克隆株ECV-p50,为研究dynei-n-dynactin复合体在登革病毒逆行性运输中的作用奠定基础。方法将构建的pRe-p50质粒稳定转染至ECV304细胞,通过间接免疫荧光染色和免疫印迹实验进行鉴定。结果经间接免疫荧光染色和免疫印迹实验验证,稳定过表达p50基因的细胞克隆株命名为ECV-p50。结论成功建立了稳定过表达p50基因的细胞克隆株ECV-p50,为后续研究积累了有价值的实验资料。 Objective To construct stable pS0 gene overexpression cells for studying the role of dynein-dynactin in the retrograde transport of Dengue virus. Methods ECV304 ceils were stable transfected by pRe-p50. Then the stable pS0 gene overexpression cells were con- firmed by western blot and indirect immunofluorescence assay. Results The stable p50 gene overexpression cells were selected and identified,named ECV-p50. Conclusion The stable p50 gene overexpression ceils have been constructed successful, which lays a base for further study on roles of p50 in virus infection.
作者 陈炜 高娜 王嘉丽 张俊磊 田衍平 安静
机构地区 第三军医大学基础医学部微生物学教研室重庆市微生物工程实验室 第三军医大学基础医学部组织胚胎学教研室 首都医科大学基础医学院微生物学教研室
出处 《局解手术学杂志》 2009年第2期78-80,共3页 Journal of Regional Anatomy and Operative Surgery
基金 国家自然科学基金项目(30471552)
关键词 病毒 微管骨架 逆行性运输 过表达 P50 virus microtubules retrograde transport overexpression p50
分类号 R373.33 [医药卫生—病原生物学]
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参考文献11

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局解手术学杂志
2009年 第2期

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