莫西沙星对流感嗜血菌生物被膜内细菌的清除作用

Eradication of Haemophilus influenzae in Biofilms in vitro by Moxifloxacin

目的探讨不同浓度莫西沙星对体外流感嗜血菌生物被膜(BBF)的破坏作用。方法体外建立流感嗜血菌生物被膜,通过结晶紫法及菌落计数观察不同浓度莫西沙星对流感嗜血菌生物被膜的破坏作用。结果莫西沙星随药物浓度的升高吸光度值逐渐降低,>4 mg/L浓度的药物可明显减轻BBF;随着药物浓度的增高,BBF内的活菌数也逐渐减少,>0.25 mg/L浓度膜内活菌数明显减少。结论莫西沙星可破坏生物被膜并能减少膜内活菌数。

OBJECTIVE To determine the activity of moxifloxacin on bacterial biofilms formed by Haemophilus influenzae in vitro. METHODS Formation of bacterial biofilm was examined by crystal violet assay, viable cells counting in biofilms was also carried out. Alterations of biofilms were measured under varying concentrations of moxifloxacin. RESULTS Optical density values of biofilms were significantly decreased at the concentrations higher than 4 mg/L of moxifloxacin. The similar results were obtained for viable cell counting. Bacteria in biofilms were eliminated partly or completely at concentrations higher than 0.25 mg/L. CONCLUSIONS Moxifloxacin is able to destroy the biofilms and reduce viable cells counting at high concentrations.