血清HBV NRAg的临床应用探讨

Detecting Nucleic Acids Related Antigen of Hepatitis B Virus: A Clinical Study

目的探讨乙型肝炎病毒核酸相关抗原(HBV NRAg)用于临床乙型肝炎诊断的实验价值及与乙型肝炎病毒DNA定量的相关性。方法收集门诊及住院患者标本432份,ELISA法检测血清HBV NRAg、HBV血清标志物,FQ-PCR检测HBV DNA,同时进行肝功能生化检测。结果HBV NRAg与HBV DNA的总符合率为90.70%;86例HBV DNA阳性样本中,HBV NRAg阳性率为95.35%,显著高于PreS1(79.06%)和HBeAg(51.16%),差异有统计学意义(P<0.05)。结论HBV NRAg与HBV DNA具有很好的一致性,可作为临床HBV感染的筛选及判断HBV复制的有意义的补充项目。

OBJECTIVE To detect situation of HBV NRAg, and discuss the correlation of the HBV NRAg with HBV Marker and HBV DNA loads, respectively. METHODS 432 serum samples were collected from the patients of Guizhou Provincial People＇s Hospital,and HBV NRAg and HBVM were detected by using ELISA. At the same time,the HBV DNA loads was examined by FQ-PCR. Statistical analyses were performed to analysis the correlation of HBV-NRAg with HBV M and HBV DNA, respectively. RESULTS A good conformability was observed between the test results of HBV NRAg and HBV DNA（90.70%）. The positive rate of HBV-NRAg in 86 HBV DNA positive samples was 95. 35%, which was higher than that of PreS1 （79. 06%） and HBeAg （51.16%）. CONCLUSIONS HBV NRAg can be seen as a good evaluation index in terms of the evaluation on replicating phase HBV and its infectivity after being infected.