聚合酶链反应在嗜肺军团菌性肺炎诊断中的应用

Rapid Diagnosis of Legionella pneumophial Pneumonia Used Polymerase Chain Reaction

目的探讨聚合酶链反应在嗜肺军团菌肺炎快速诊断中的应用价值。方法53例呼吸系感染病原不明性肺炎住院患者,采集痰液标本47份,支气管灌洗液标本6份,应用聚合酶链反应检测嗜肺军团菌DNA(LPN-DNA)。结果53例病原不明性肺炎患者检出LPN-DNA阳性5例,总阳性率为9.4%,其中47份痰液标本检出LPN-DNA阳性3例(6.4%),6份支气管洗液标本检出LPN-DNA阳性2例(33.3%)。结论聚合酶链反应检测临床标本中LPN-DNA诊断病原不明性肺炎具有良好的临床实际应用价值。

OBJECTIVE To prove the diagnosis value for Legionnaires pneurnophial pneumonia using polymerase chain reaction. METHODS L. pneumophial-DNA （LPN-DNA） from 47 spuum and 6 bronchoalveolar lavage fluid samples collected from 53 patients with atypical pneumonia was detected by PCR. RESULTS The positive rate of LPN-DNA in 53 patients with atypical pneumonia was 9. 4%, while the positive rate of sputum and bronchoalveolar iavage fluid samples was 6.4%and 33.3%, respectively. CONCLUSIONS LPN-DNA detected by PCR for early diagnosis of atypical pneumonia has favorable clinical application.