摘要
目的：建立广藿香紫苏叶挥发油微乳中百秋李醇的GC含量测定方法。方法：采用挥发油提取器从微乳中提取挥发性成分，用GC法测定挥发油微乳中百秋李醇的含量。色谱条件：HP．5色谱柱；柱温采用程序升温；气化温度为270℃；检测器温度为280℃；分流比为20：1；流速为1mL·min^-1。结果：表明百秋李醇在0．4064-4．8768弘g范围内呈良好的线性关系，回归方程为y=0．58904X-0．0214，r=0．9998，平均回收率为97．10％（n=5）。结论：方法准确可靠，重复性好。可用于挥发油微乳中百秋李醇的含量测定。
Objective：To determine the content of patchouli alcohol in microemulsion of volatile oils of Cablin Patchouli Herb and Perilla Leaf by GC. Methods： HP-5 column was used. Column temperature was controlled by a temperature programm. The injector temperature was 270 ℃ and the detector temperature was 280 ℃. The flow rate of carrier gas was 1 mL·min^- 1 . Results ： A good linearity of patchouli alcohol was found over the injection a mounts of 0.406 4μg - 4.876 8μg. The regression equation was Y = 0.589 0X - 0.021 4（ r = 0.999 8） . The average recovery was 97.10% with a RSD of 1.23%. Conclusion： The method is simple and accurate and can be used as a quality control method for the microemulsion of volatile oil of Cablin Patchouli Herb and Perilla Leaf.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第4期4-6,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
十一五国家科技支撑计划(2006BAI09808-15)
国家自然科学基金(30472195)
关键词
气相色谱法
微乳
广藿香油
紫苏叶油
百秋李醇
GC
microemulsion
patchouli alcohol
volatile oil
Cablin Patchouli Herb
Perilla Leaf
