大鼠脑出血后高血糖对脑损伤的实验性研究

The neurocyte injury mechanism experimental research of hyperglycemia after cerebral hemorrhage in rats

目的:观察大鼠脑出血(ICH)后高血糖对大鼠血肿周围脑组织含水量、血糖、乳酸(LA)等指标的影响,探讨ICH后高血糖对脑损伤的可能机制。方法:自体血注入法建立脑出血模型,将SD大鼠80只随机分成假手术组、单纯ICH组、ICH后高血糖组,最后一组又分为2g/kg注糖组和4g/kg注糖组。在相应时点监测血糖,各组大鼠于相应时点迅速处死并取脑,测定血肿周围脑组织含水量以及乳酸的含量。结果:单纯ICH组术后血糖明显高于假手术组(P<0.05);高血糖两组血肿周围脑组织含水量和乳酸含量明显高于单纯ICH组(P<0.05),以高剂量注糖组升高更明显(P<0.05)。结论:提示脑出血后可能存在应激性血糖升高,脑出血后高血糖使乳酸蓄积可能加重脑水肿,加重脑损伤。

Objective： To observe the damage of experimental cerebral hemorrhage of rats and the possible mechanism. Methods： Horseley-Clarke technique, autologous blood or saline （50？ 1 each） was injected into the striatum of Sprague-Dawley rats to make intracerebral hemorrhage models. Adult male SD rats were used. The rats were randomly divided into four groups： sham operation group, cerebral hemorrhage group and cerebral hemorrhage with hyperglycemia group （hypertonic glucose 2g/kg,4g/kg）. The brain water content was detected by dry wet weigh method . The activities of LA at different time were measured with spectrophotometry. Results： Serum glucose at various stages in cerebral hemorrhage were higher than those in sham operation after operation （P〈0. 05）;Brain water contents and the activities of LA at various stages in hypertonic glucose （2g/kg,4g/kg,ip） treatment groups were lower than those in cerebral hemorrhage group （P 〈0. 05）; Brain water contents and the activities of LA at various stages in hypertonic glucose （4g/kg, ip） treatment group were lower than those in hypertonic glucose （2g/kg, ip）treatment group （P（0.05）Conclusion： There is Stress hyperglycemia after cerebral hemorrhage, lactic acid accumulation may aggravate brain injury after ICH.