姜黄素对抑郁症大鼠模型行为学及神经肽Y的影响

Influence of curcumin on ethology and neuropeptide Y in rat model of depression

目的姜黄素的抗抑郁样作用及对慢性应激抑郁大鼠模型海马、下丘脑、血浆中神经肽Y(NPY)含量的影响。方法SD雄性大鼠24只,用随机区组设计的方法分为对照组、模型组、盐酸氟西汀组、姜黄素组,处理21 d后,观察1%蔗糖水摄取率和强迫游泳不动时间,用竞争酶联免疫分析法测定NPY含量。结果在处理21 d后,姜黄素组、氟西汀组大鼠与模型组大鼠比较,强迫游泳不动时间明显缩短(P<0.05),1%蔗糖水摄取量明显升高(P<0.01)达到对照组水平;在处理21 d后,正常组、姜黄素组、氟西汀组大鼠与模型组大鼠比较,海马、下丘脑NPY含量变化无统计学差异;在处理21 d后,对照组大鼠血浆NPY含量与模型组有显著差异(P<0.01),氟西汀组、姜黄素组、模型组大鼠血浆NPY含量无统计学差异。结论姜黄素具有抗抑郁样作用,其效果与一线抗抑郁药盐酸氟西汀相仿。本次结果尚不足以证明姜黄素可以通过调节NPY含量发挥抗抑郁作用,其抗抑郁样作用尚需进一步研究。

Objective To study the anti-depression effect of curcumin and its influence on neuropeptide Y（NPY） contents in the hippocampus,hypothalanus and blood plasma in rats with chronic stress depression.Methods All SD male rats（n=24） were divided into the control group,model group,fluoxetine hydrochloside（HCI） group and curcumin group.After 21 days the taking rate of 1% sugar water and forced swimming standing time were observed and NPY contents were detected by ELISA.Results After 21 days the forced swimming standing time was significantly shortened（P〈0.05） and the taking rate of sugar water was significantly increased to the same level of the control group（P〈0.01） in the curcumin group and fluoxetine HCI group than those in the model group.After 21 days NPY contents in the hippocampus and hypothalanus had no significant statistical difference between the normal group,curcumin group or fluoxetine HCI group and model group.After 21 days NPY contents in blood plasma had significant difference between the control group and model group（P〈0.01）.There was no statistical difference in plasma NPY content among fluoxetine HCI group,curcumin group and model group.Conclusion Curcumin has anti-depression effect that is similar to that of fluoxetine HCI.The result of this study has not proved the anti-depression effect of curcumin through regulating NPY contents,so further studies are needed.