乳癖舒对乳腺增生病大鼠的治疗作用

Therapeutic effect of Rupishu Decoction on cyclomastopathy in rats

目的通过观察乳癖舒对乳腺增生大鼠性激素和乳腺组织的影响,从而了解乳癖舒对乳腺增生大鼠的治疗作用。方法采用苯甲酸雌二醇和黄体酮肌注造模30 d后,分别用三苯氧胺、逍遥散和乳癖舒治疗30 d,同时继续肌注雌二醇。采用放射免疫法测定血清中雌二醇(E2)和孕酮(P)含量。取乳房组织行HE染色,光镜观察病理变化。结果乳癖舒、三苯氧胺、逍遥散可减少乳腺增生病大鼠乳腺小叶腺泡数,缩小腺泡腔和小叶导管直径;并使血清E2水平和雌二醇/孕酮比例(E2/P)降低。结论乳癖舒可通过调节乳腺增生大鼠的性激素水平,改善增生乳腺的形态学变化而有效对抗雌激素引起的大鼠乳腺增生。

Objective To discuss the therapeutic effect of Rupishu Decoction on cyclomastopathy through observing the influence of Rupishu Decoction on sex hormones and mammary glands in rats with cyclomastopathy.Methods The model was established by injecting benzoate estradiol and progestogen in rats for 30 days,and then the rats were treated with tamoxifen,Xiaoyao Powder and Rupishu Decoction respectively and injected estradiol（E2） at the same time for 30 days.The pathological changes of rat mammary glands were observed with the optical microscope after HE staining,and the levels of E2 and progestogen（P） were detected by radioimmunoassay.Results Rupishu Decoction,tamoxifen and Xiaoyao Powder reduced the quantity of the lobule-alveolus of mammary glands,decreased the lobule-alveolus cavity and diameter of the interlobular ducts,and decreased the level of E2 and ratio of E2 to P.Conclusion Rupishu Decoction can inhibit mazoplasia induced by sex hormones through regulating the sex hormone level and relieving the histological changes of mammary glands in rats with cyclomastopathy.