Roscovitine对增生期肝癌细胞周期的影响

Roscovitine on cell cycle in mitotic hepatoma carcinoma cell

目的探讨细胞周期蛋白依赖激酶抑制剂Roscovitine对增生期肝癌细胞SMMC-7721细胞周期的影响。方法采用体外培养的肝癌细胞SMMC-7721，经过Roscovitine作用后，对SMMC-7721细胞的形态、生长情况、细胞周期时相的分布、凋亡以及CDK2、Caspase-3、bcl-2mRNA的表达情况进行观察。结果MTT提示：Roscovitine对SMMC-7721细胞的增生有抑制作用，作用效果呈时间、剂量依赖性，并促进细胞的凋亡；流式细胞仪发现G0期、G1期的比例增加，细胞出现凋亡；R-T PGR显示CDK2mRNA表达水平降低，凋亡相关基因bcl-2表达降低，Caspase-3mRNA水平表达升高。结论Roscovitine可以抑制增生期肝癌细胞的生长、增生，阻滞细胞周期于G0／G1期，诱导细胞的凋亡，凋亡机制与bcl-2、Caspase-3的表达改变有关。

Objective To study the influences by a Cyclin-dependent kinase inhibitor Roscovitine on cell cycle in mitotic hepatoma carcinoma cell SMMC-7721. Methods Microscope,MTT, flow cytometry and R-T PCR were used to observe the effects of Roscovitine on morphology, proliferation, cell cycle, apoptosis and the mRNA expression of CDK2, Caspase-3, bcl-2 in SMMC-7721 cells. Results Roscovitine inhibited the proliferation of SMMC-7721 cells in dosage and time dependent manner and induced apoptosis. Flow cytometry showed the ratio of G0, G1 increased. R-T PCR showed that the expression of bcl-2 reduced, Caspase-3 increased. Conclusion Roscovitine can inhibit the growth, proliferation, block the cell cycle at G0/G1 and promotes apoptosis of mitotic SMMC-7721 cells, and the mechanism of apoptosis is dependent on the activity of bcl-2 and Caspase-3.