行为学训练对海马损伤梗死大鼠齿状回区BrdU和Nestin表达的影响

The Effect of Behavior Training on the Expression of BrdU and Nestin in the Dentate Gyrus of Rats with Hippocampal Impairment and Infarction

目的探讨行为学训练对海马损伤梗死大鼠齿状回区神经干细胞增殖的影响。方法采用光化学法制成单侧海马损伤梗死模型大鼠72只，随机分为训练组（n=36）和自由活动组（n=36），每个组设1、7、14、21、28及35d 6个亚组。另设正常对照组56只，与模型组对应分为1、7、14、21、28及35d 6个亚组。训练组大鼠于追模1d后给予水迷宫训练，自由活动组大鼠自由活动，不予水迷宫训练。免疫荧光双标记法观察各不同时间点大鼠海马齿状回区溴脱氧尿嘧啶核苷（Bromodeoxyuridine，BrdU）与巢蛋白（Nestin）的双标记表达情况。结果正常对照组大鼠海马齿状回区有少量Brdu／Nestin双标记阳性细胞，训练组及自由活动组大鼠在7、14、21及28d海马损伤梗死侧齿状回区BrdU／Nestin双标记阳性细胞数量均有显著增多（P〈0．01）；训练组大鼠7、14、21、28d时海马损伤梗死侧齿状回区的BrdU／Nestin双标记阳性细胞数量显著高于自由活动组（P〈0．01）；至35d时，训练组及自由活动组大鼠海马损伤梗死侧齿状回区BrdU／Nestin双标记阳性细胞数量与正常对照组无明显差异（P〉0．05）。结论行为学训练能显著增强海马损伤梗死大鼠齿状回区神经干细胞的增殖，促进神经功能恢复。

Objective To explore the effect of behavior training on the proliferation of neural stem cells in the dental gyrus （DG） of hippocampus injury-infarcted rats. Methods One hundred and eight Sprague-Dawley rats were randomized into behavior training group （1, 7, 14, 21, 28 and 35 days）, no-training group （1, 7, 14, 21, 28 and 35 days） and control group （1, 7, 14, 21, 28 and 35 days）. Photochemical initiation was used to induce hippocampal injury- infarction in training and no-training group. Morris water maze training or no-training was performed 1 day after surgery. Double staining immunofluorescence was used to detect the expression of BrdU/ Nestin in the dental gyms （DG） at different time points. Results Few BrdU/Nestin double staining cells were observed in the DG of control rats. In the behavior training and no-training groups for 7, 14, 21 and 28 days, the number of BrdUfNestin double-stained cells increased in the DG on the opposite side compared with the control group （P〈0.01）. BrdU/Nestin double-stained cells increased obviously after 7, 14, 21 and 28 days in the behavior training group compared with the no-training group（P〈0.01）. In the behavior training and no-training groups for 35 days, no significant difference of the number of BrdU/Nestin double-stained cells in the DG on the opposite side was found between them and the control group （P〉0.05）. Conclusion Behavior training can accelerate the proliferation of neural stem cells and then encourage the recovery of neural function.