山莨菪碱及其大鼠肠内菌体外代谢物的液相色谱-质谱法分析

Analysis of anisodamine and its in vitro metabolites in rat intestinal flora by LC-MS^n

目的用液相色谱-电喷雾离子阱串联质谱联用法研究山莨菪碱在大鼠肠内菌中的代谢。方法以山莨菪碱优化色谱及质谱条件,总结其色谱及质谱行为规律。将山莨菪碱与大鼠肠内菌体外厌氧温孵培养,并与空白样品及山莨菪碱对照品进行比较,依据被测物的多级质谱数据,鉴定代谢物并阐述其结构。结果在温孵液中发现了山莨菪碱的脱水及水解代谢产物,即脱水山莨菪碱、6β-羟基托品和托品酸。结论该方法灵敏、快速、简单,适合于药物代谢分析。

Objective Anisodamine and its metabolites in rat intestinal flora were analysed by liquid chromatography-tandem mass spectrometry （LC-MS^n）. Methods The chromatographic and mass spectrometric conditions were optimized using anisodamine standard, The chromatographic and mass spectrometric behavior patterns of anisodamine were summarized. Anisodamine was incubated together with rat intestinal flora in vitro under lack of oxygen condition. Analysis and structural identification of the metabolites were performed by comparing their changes in molecular masses, retention-time, and full scan MS^n spectra with those of the parent drug and blank plasma. Results The results revealed that the parent drug and its dehydrolyzed and hydrolyzed metabolites （apoanisodamine, 6β-hydroxytropine, and tropic aeid） were found in the incubation solution. Conclusion This method is sensitive, rapid, and simple, and suitable for the analyses of drugs metabolites.