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大肠埃希菌对氨基糖苷类抗生素的耐药性及耐药基因检测 被引量：2

Detection of Aminoglycosides Resistance and Resistance Gene of Escherichia coli
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摘要 目的:了解大肠埃希菌对氨基糖苷类抗生素的耐药情况与耐药基因携带情况。方法:使用K-B法检测大肠埃希菌对氨基糖苷类抗生素的耐药性,同时用PCR技术扩增氨基糖苷类抗生素的耐药基因以明确其基因型。结果:40株大肠埃希菌中耐氨基糖苷类抗生素菌株32株,耐药率为80.0%(32/40),其中aac(3)-Ⅰ 2株,占6.3%(2/32),aac(3)-Ⅱ 28株,占87.5%(28/32),aac(6′)-Ⅰ 12株,占37.5%(12/32),aac(6′)-Ⅱ 8株,占25%(8/32),ant(2″)-Ⅰ 4株,占18.8%(6/32),ant(3″)-Ⅰ 9株,占28.1%(9/32)。结论:耐氨基糖苷类抗生素的大肠埃希菌比较普遍,氨基糖苷类耐药基因以aac(3)-Ⅱ和aac(6′)-Ⅰ为主。 Objective：To know the aminoglycosides resistance and resistance gene of Escherichia coli.Method：K-B test was used to detect the drug resistance of Escherichia coli to antibiotics and PCR was used to amplify aminoglycosides resistance gene.Results：32 strains were resistant to aminoglycosides of 40,drug-resistancerate 80.0%.2 strains of aac（3）-Ⅰ,28 of aac（3）-Ⅱ,12 of aac（6′）-Ⅰ,8 of aac（6′）-Ⅱ,4 of ant（2″）-Ⅰ,9 of ant（3″）-Ⅰ.Conclusion：The Escherichia coli of aminoglycosidesresistance is relatively high,aac（3）-Ⅱ and aac（6′）-Ⅰare the most capital genotypes in Escherichia coli of aminoglycosides-resistance.
作者 于丽萍 韩文清 姜筱冰
机构地区 青岛大学医学院附属烟台毓璜顶医院 烟台市疾病预防控制中心
出处 《中国误诊学杂志》 CAS 2009年第13期3033-3035,共3页 Chinese Journal of Misdiagnostics
关键词 大肠杆菌/药物作用 抗药性 细菌/遗传学 氨基糖苷类/药理学 Escherichia coli/drug effects Drug Resistance,Bacterial/genetics Aminoglycosides/pharmacology
分类号 R978.1 [医药卫生—药品]
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共引文献621

同被引文献23

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中国误诊学杂志
2009年 第13期

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