摘要
本文利用线扫极谱法研究了镉（Ⅱ）-邻菲啰啉（Phen）二元络合物与透明质酸（HA）的相互作用并建立了以Cd（Ⅱ）-Phen二元配合物为电化学探针测定HA的分析测试方法。在最佳实验条件下，cd（Ⅱ）一Phen还原峰电流的降低值同HA的浓度在4．0～110．0mg／L范围内呈良好的线性关系，线性回归方程为Δip”（nA）=3．98C（mg／L）＋30．79（n=17，γ=0．993），检测限为3．23mg／L（3σ）。将本方法应用于模拟样品中HA的测定，结果令人满意。
In this work, the interaction of Cd （ Ⅱ ） -Phen with HA was investigated by linear sweep polarographic method, accordingly a new approach for HA determination using Cd（ Ⅱ ） -Phen as an electrochemical probe was established. Under the optimal conditions, the reduction the HA concentration in the range of 4.0- 110.0 mg/L, peak current of Cd（Ⅱ） - Phen decreases proportional to the linear regression equation was got as Δip （ hA） = 3. 98 C （mg/L） ＋30.79 （n =17, γ =0.993） with the detection limit as 3.23 mg/L （3σ）. The proposed method was further applied to determine the HA content in the synthetic samples with good results and good recovery.
出处
《青岛农业大学学报（自然科学版）》
2009年第1期66-69,共4页
Journal of Qingdao Agricultural University(Natural Science)
