摘要
用响应面法对茶薪菇产纤维素酶的发酵条件进行了优化。首先用Plackett-Burman法筛选出三个影响较大的重要因素,分别为:碳源、初始pH值和发酵时间,然后进行最陡爬坡实验逼近最佳响应面区域,最后通过Box-Behnken设计,利用SAS软件进行回归分析,得到各因素的最佳水平。在优化的培养条件下,纤维素酶的产量提高约45.26%。
Response surface analysis was applied to optimize the fermentation conditions for cellulase production by Agrocybe chaxingu. Firstly, two-level factorial design of Plackett-Burman was used to evaluate the effect of seven related factors on produced cellulase activity, and three main factors were screened out, including carbon source, pH value and fermentation time. Then the path of steepest ascent was taken to approach the optimal region of fermentation conditions. Finally, the optimum levels of three main factors were obtained through Box-Behnken design, regression analysis with statistical analysis system and response surface analysis. Through the optimization of culture conditions, the cellulase activity is promoted by 45.26%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第7期162-165,共4页
Food Science
基金
鲁东大学校基金项目(20053305)
关键词
SAS
响应面
茶薪菇
纤维素酶
statistical analysis system （SAS）
response surface
Agrocybe chaxingu
cellulase
