摘要
本实验以低能氮离子和紫外线作为诱变手段对乳链菌肽产生菌进行诱变,筛选出一株优良菌株Lactococcus lactisK25,发酵效价由初始的1732.45IU/ml提高到2291.75IU/ml,提高了32.3%。遗传稳定性实验结果表明,Lactococcus lactisK25遗传性状稳定良好。
In this study, the nisin-producing strain Lactococcus lactis J7 was mutagenized using low energy N^＋ implantation and UV radiation, and its mutant strain Lactococcus lactis K25 was obtained. The nisin potency is increased from 1732.45 IU/ ml to 2291.75 IU/ml, by 32.3% through the mutagenesis ofLactococcus lactis J7. Moreover, Lactococcus lactis K25 shows nice genetic stability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第7期177-180,共4页
Food Science
基金
国家"973"计划项目(2004CB719604)
关键词
乳链菌肽
离子束注入
诱变
nisin
ion beam implantation
mutagenesis
