免疫初乳对腹泻大鼠血液免疫细胞活性和免疫介质调节作用研究

Effects of Immune Colostrum on Blood Immunocyte Activities and Immune-mediator Regulation in Diarrhea Rats

目的:探讨免疫初乳在干预急性感染性腹泻病中的保护效应及其机制。方法:选用32只健康成年SD大鼠,随机分成对照组(C组)、模型组(M组)、普通初乳保护组(NC组)、免疫初乳保护组(IC组)。NC和IC组每天分别灌服普通初乳和免疫初乳(1ml/100gbw),C和M组灌服生理盐水,连续12d。在灌服实验后的第5、10d,M、NC和IC组大鼠(1ml/100g)感染混合的肠侵染性大肠杆菌和沙门氏菌菌液(108/ml)。结果:M和NC组大鼠血液红细胞CR1(RBC-CR1)花环率和红细胞免疫复合物(RBC-IC)花环率分别显著低于和高于C组,而IC组和C组无显著差异;M和NC组T淋巴细胞比例显著下降,但IC组与C组无明显差异;感染腹泻病原菌导致血浆IL-2水平下降,TNF-α水平升高,但IC组和C组相近。结论:免疫初乳可以通过改善和提高血液免疫细胞功能活动,介导免疫介质的调整作用发挥抗大鼠感染性腹泻的保护效应。

Objective： To discuss the protective effect and its mechanisms of immune colostrum in intervening in acute infectious diarrhea disease. Methods： Thirty-two healthy adult SD rats （half females and half males） were chosen and randomly divided into a control group （group C）, a model group （group M）, a group protected with normal colostrum （group NC） and a group protected with immune colostrum （group IC）. Rats in groups NC and IC were orally given normal colostrum and immune colostrum at a dosage of 1 ml per 100 g body weigh once each day for 12 days, respectively. The animals in groups C and M were received physiologic saline at the same dose and frequency. On days 5 and 10 post experiment, animals from groups M, NC and IC were orally infected （1 ml/100 g body weight）with mixed enteroinvasive E. coli and Salmonella typhi （108/ml）. Results： During the whole experimental period, red blood cell complement receptor type 1 （RBC-CR1） rosette rates and red blood cell immune complex （RBC-IC） rosette rates in groups M and NC are obviously lower and higher than those in group C, respectively, while there is no significant difference between group IC and group C. The proportions of T-lymphocytes in groups M and NC significantly reduce, but no obvious difference is observed between group IC and group C during the whole experiment period. The decreased IL-2 and elevated TNF-α levels are detected in plasma of diarrhea pathogens-infected rats, but the levels of IL- 2 and TNF- α in group IC are close to those in group C. Conclusion： Immune colostrurn provides effective protection of antiinfectious diarrhea in rats via ameliorating and enhancing activities and function of blood immunologic cells as well as mediating regulation of immune-mediators.