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16S rRNA序列分析及其在乳酸菌分类、鉴定中的应用 被引量：13

Sequence Analysis of 16S rRNA and Its Application in Classification and Identification of Lactic Acid Bacteria
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摘要 综述16SrRNA的特点及序列分析的基本方法,并详细介绍了几种在乳酸菌分类和鉴定中较常用的16SrRNA相关的分子生物学方法,如16SrRNA直接测序、寡核苷酸探针、限制性片段长度多态性分析、温度梯度凝胶电泳等,以及这些方法的研究进展。 The characteristics of 16S rRNA and the fundamental methodology of its sequence analysis were reviewed in this paper. Several molecular biological methods related to 16S rRNA sequence analysis were detailed to classify and identify lactic acid bacteria such as direct sequencing, oligonucleotide probes, the polymorphism of restriction fragment length, temperature gradient gel electrophoresis. The recent progresses in the application of these methods were also introduced in detail.
作者 赵玉娟 牛春华 张雪 李达 张健 杨贞耐
机构地区 吉林省农业科学院农产品加工中心
出处 《食品科学》 EI CAS CSCD 北大核心 2009年第7期299-303,共5页 Food Science
基金 科技部国际合作项目(2006DFA32620) 吉林省科技厅重点项目(20060219)
关键词 乳酸菌 16S RRNA 分类 鉴定 lactic acid bacteria 16S rRNA classification identification
分类号 Q939.1 [生物学—微生物学]
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参考文献33

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二级参考文献9

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引证文献13

二级引证文献79

食品科学
2009年 第7期

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