响应面法优化蜂胶超临界二氧化碳萃取工艺的研究

Optimization of Supercritical Carbon Dioxide Extraction of Effective Components from Propolis Using Response Surface Methodology

采用响应面法优化蜂胶超临界二氧化碳萃取工艺,根据中心旋转试验设计,研究萃取压力、萃取温度和二氧化碳流速等工艺条件对萃取率的影响。结果表明,蜂胶萃取物的萃取率随温度的升高而降低;随压力和二氧化碳流速的增加萃取率不断增大,而在高压和高流速下又呈现下降趋势。分析所得的回归方程确定最佳工艺条件为:萃取压力33.4MPa,温度45.4℃,二氧化碳流速16.8L/h。在此条件下萃取蜂胶120min时萃取物的萃取率达19.62g/100g蜂胶。GC-MS分析表明萃取物中含有较高含量的烷烃类物质和烷醇类物质,以及肉桂酸肉桂酯、柯因、5-甲基柯因等。

Response surface methodology （RSM） was employed to optimize the supercritical CO2 extraction conditions of effective components from propolis. The effects of pressure, temperature and CO2 flow rate on the yield were investigated. Results showed that the yield decreases with the rise of extraction temperature, while it firstly increases with the increase of pressure and COL flow rate until certain high levels of pressure and COL flow rate and then begins to decrease. Moreover, the optimum pressure, temperature and CO2flow rate are 33.4 MPa, 45.4 ℃ and 16.8 L/h, respectively. Under such conditions, the yield reaches 19.62 g/100 g propolis after extraction for 120 min. The GC-MS analysis indicated that the main components in the extract are alkanes, cosanols, cinamyl cinnamate, chrysin and chrysin 5-methyl ester.