胡芦巴中4-羟基异亮氨酸的提取分离工艺研究

Study on Extraction and Purification of 4-Hydroxyisoleucine from Trigonella foenungraecum

优化4-羟基异亮氨酸提取纯化工艺。以甘肃天水产胡芦巴为原料,在单因素试验基础上选取料液比、提取时间、提取温度为影响因素,进行均匀设计优化,结果显示最佳提取工艺为:10倍量水在90℃下提取2.0h,共提取2次。对提取浓缩液进行了纯化,最佳工艺为:5倍量90%乙醇沉淀、732型树脂吸附、pH9的氨水在流速为0.6ml/min下洗脱,洗脱液总用量800ml,得到的4-羟基异亮氨酸含量可达40%。该工艺较先进,可应用于工业化生产。

the extraction conditions of 4-hydroxyisoleucine from Trigonellafoenungraecum planted in Tianshui of Gansu province were optimized by uniform design based on single factor tests of several factors such as ratio of extractant to material, extraction time, temperature and extraction times. The results showed that the optimum ratio of water to material, extraction time, temperature and extraction times are 10：1 （ml/g）, 2 h, 90 ℃ and twice, respectively. After concentration, the water extract of Trigonellafoenungraecum was added with 90% ethanol, and then the mixture was centrifugalized to remove Trigonellafoenungraecum gum. Subsequently, the supernatant was adsorbed with 732 strong acid styrene cation exchange resin. The desorption conditions of 4-hydroxyisoleucine were investigated. The results showed that the optimal addition amount of 90% ethanol is 5 times of volume of water extract of Trigonellafoenungraecum. The optimal desorption conditions are as follows： ammonia water pH 9, its total amount for desoprtion 800 ml, and its flow rate for desorption 0.6 ml/min. Under the above conditions, the content of 4-hydroxyisoleucine in final product is about 40%. After used repeatedly 5 times, the resin needs regenerating.