大叶白麻不同部位总黄酮和金丝桃苷含量研究

Determination of Total Flavonoid and Hyperoside Contents in Different Parts of Poacynum hendersonii (Hook.f.) Woodson

本研究采用高效液相色谱法(HPLC)测定了大叶白麻叶、花、根和茎四个部位的金丝桃苷含量;以NaAc-A1C13体系为显色剂,用分光光度法于400nm处测定了大叶白麻不同部位总黄酮的含量。结果表明:本研究采用的色谱条件可用来检测大叶白麻体内金丝桃苷。叶中的金丝桃苷含量较高,约为0.24%,花、茎和根中几乎检测不出;叶中的总黄酮含量亦较高,约为3.54%,花、茎和根中含量较低。

The hyperoside contents in different parts of Poacynum hendersonii （Hook.f.） Woodson were measured by HPLC method, and the total flavoniod contents were determined by spectrophotometry at 400-nm wavelength. The results showed that the hyperoside content in the leaves is higher, about 0.24%, while almost no hyperoside is found in the flowers, stems and roots. Similarly, the total flavonoid content in the leaves is also higher, about 3.54%, while that is lower in the flowers, stems and roots.