离子辐照对生防菌BJ1的诱变研究及菌种鉴定

Studies on the effects of ^(12)C^(6+) ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1

为了提高生防菌BJ1的抑菌能力,优化辐照诱变的物理参数,获得生防效果更好的高效菌种,利用12C6+对生防菌BJ1进行不同剂量离子辐照处理。结果表明,采用100Gy剂量辐照的生防菌BJ1的存活率最高,并且在0～100Gy之间,存活率先降后升,其存活曲线呈"鞍型";从诱变效果看,经400Gy辐照诱变获得的生防菌BJ1的抑菌效果有明显提高;综合存活和抑菌效果考虑,离子辐照诱变生防菌BJ1的最适剂量为200～400Gy。通过16SrDNA序列分析,将辐照诱变获得的BJ1菌株定为枯草芽孢杆菌。

ABSTRACT Biocontrol bacterium BJ1 was irradiated with different doses of ^12C^6＋ ion beam in order to improve the antagonistic ability, to optimize the parameters of ion beam irradiation and to obtain high effect of biocontrol strains. The results show that the survival rate of BJ1 irradiated with 100 Gy reaches the highest and the survival curve of B J1 presents a tendency of going down first and then increasing, which takes a shape of ＂saddle＂ during 0 to100 Gy. In view of the effect of mutation induction, BJ1 irradiated with 400 Gy enhances its effect of antagonistic ability obviously. Based on the results of survival rate and the effect of antagonistic ability, an optimal treatment dose of ^12C^6＋ irradiation is 200 to 400 Gy for BJ1. And B J1 has been identified to be Bacillus subtilin by 16S rDNA sequence cluster analysis.