台湾生产的大、小承气汤浓缩颗粒质量分析研究

Quality analysis of Da,Xiao-Chengqi Decoction produced in Taiwan

目的：用HPLC法同时测定购自台湾的大、小承气汤浓缩颗粒中柚皮苷、大黄酸、大黄素、大黄酚、和厚朴酚及厚朴酚的含量。方法：采用Licrospher C18柱（150mm×4．6mm，5μm），以乙腈-0．5％冰醋酸梯度洗脱，检测波长：270nm，流速：1mL／min，柱温：25℃。结果：所测8个大、小承气汤样品中柚皮苷的含量在0．54—44．20mg／g；大黄酸的含量在0．51—2．10mg／g；和厚朴酚的含量在0．44—6．65mg／g；大黄素的含量在0．02-0．23mg／g；厚朴酚的含量在1．09—2．92mg／g；大黄酚的含量在0．09—2．70mg／g，但个别样品中未检出和厚朴酚及厚朴酚。此方法加样回收率与线性范围均符合要求。结论：本方法结果准确、灵敏度高、重现性好，且对台湾市售的8个样品均可适用，具有方法的普遍性，可作为大、小承气汤质量控制的定量方法。

AIM ： To establish a HPLC method for determing naringin, rhein, emodin, chrysophanol, honokiol and magnolol in Da, Xiao-Chengqi Decoction bought from Taiwan. METHODS： The seperation was performed on Licrospher C18 column （150 mm ×4.6 mm,5 μm） with acetonitrile-0.5% HAc （gradient elution） as mobile phase and the detection wavelength was set at 270 nm. RESULTS ： In the eight samples measured the content of naringin was from 0.54 to 44.20 mg/g; rhein from 0.51 and 2.10 mg/g; honokiol from 0.44 to 6.65 mg/g; emodin from 0.02 to 0.23 mg/g; magnolol from 1.09 to 2.29 mg/g, chrysophanol from 0.09 to 2.70 mg/g. However, honokiol and magnolol were not detected in some samples. CONCLUSION： This established method is fully validated with respect to linearity, precision, reproducibility and accuracy and successfully applied to quantifying the six constituents in eight commercial samples. The overall results demonstrate that this proposed method is simple, reliable, and suitable for the quality control of Da, Xiao-Chengqi Decoction.