贯叶连翘超临界提取物的高效液相色谱分析

Identification of compounds in supercritical CO_2 extraction from Hypericum Perforatum L. by HPLC

目的:对贯叶连翘CO2超临界提取物的成分进行分析,并测定其中主要化学成分的含量。方法:以高效液相色谱法分别测定超临界提取物中主要黄酮类成分和贯叶金丝桃素含量。CO2超临界提取条件:萃取温度40℃,萃取压力20MPa,分离温度为45℃,压力为8 MPa,提取1 h。结果:纯CO2超临界提取的提取物不含黄酮成分,贯叶金桃丝素含量达180.6 mg/g;以乙醇为夹带剂的超临界提取物含有黄酮类成分和贯叶金丝桃素,芦丁、金丝桃苷、槲皮素含量分别为1.10mg/g、1.84 mg/g、1.33 mg/g,贯叶金丝桃素含量为26.19 mg/g。结论:用纯CO2超临界提取,提取物中贯叶金丝桃素含量远高于加夹带剂超临界提取,可用本法制备贯叶金丝桃素含量高的提取物。

AIM ： To identify and determine compounds in supercritical CO2 extraction from Hypericum perforaturn L. METHODS： The contents of flavones and hyperforin were determined by reverse phase HPLC. The condition in supercritical fluid extraction （SFE） was： extraction temperature at 40 ℃, extraction pressure at 20 MPa, separation temperature at 45 ℃, separation presure at 8 MPa, extraction time 1 h. RESULTS： There was no flavanes and the content of hyperforin was 180.6 mg/g in the sample of SFE using neat CO2. The flavones and hyperforin were found in SFE extract using ethanol as modifier and the contents of turin, quercetin, hyperoside, hyperforin were 1.10 mg/g,1.84 mg/g,1.33 mg/g,26.19 mg/g, respectively. CONCLUSION： The concentration of hyperforin by SFE with neat CO2 is significantly higher than that with modified supercritical CO2 extraction. The method of SFE with neat CO2 can be used to prepare extracts with high concentration hyperforin.