低氧诱导因子-1α和Survivin在宫颈鳞癌组织中的表达及意义

Expressions and sifnificances of HIF-1αand Survivin in cervical squamous cell carcinoma

目的探讨低氧诱导因子-1α(hypoxia-inducible factor-1α,HIF-1α)和Survivin在宫颈鳞癌中的表达及其临床意义。方法采用免疫组化SP法检测50例宫颈鳞癌及10例正常宫颈组织中HIF-1α和Survivin蛋白的表达情况,并结合临床病理特征进行分析。结果(1)HIF-1α和Survivin在宫颈鳞癌中的表达阳性率分别为64%、70%,明显高于正常对照组(二者在正常宫颈组织中均无表达),P<0.05,差异有显著性。(2)HIF-1α与临床分期显著相关(χ2=4.67,P<0.05),与分化程度显著相关(χ2=9.04,P<0.05);Survivin与临床分期显著相关(χ2=5.36,P<0.05),与分化程度有明显相关性(χ2=6.32,P<0.05)。(3)HIF-1α和Survivin在宫颈鳞癌中阳性表达呈正相关关系。结论HIF-1α与Survivin均在宫颈鳞癌的发生、发展中起重要作用,且两者在宫颈鳞癌的演进中可能起着协同作用,故联合检测可作为判断宫颈鳞癌恶性程度及评估预后的重要指标。

Objective To investigate expressions and signifieances of HIF-1α and Survivin in cervical squamous cell carcinoma. Methods The expressions of HIF-1α and Survivin in 50 cases of cervical squamous cell carcinoma,10 cases of normal cervical tissues were detected witb SP method of immuohistochemistry.Results （1）The expressions of HIF-1α and Survivin in cervical squamous cell carcinoma were 64%,70% respectively, but were 0% in normal cervical tissues, there were significant differences （P〈0.05）. （2）The expression of HIF-1α was significantly correlated with clinical stage of cervical squamous cell carcinoma （X^2=4.67,P〈0.05）;differentiation degree of tumor cells （X^2=9.04,P〈0.05）;The expression of Survivin was significantly correlated with clinical stage of cervical squamous cell carcinoma（X^2=5.36,P〈0.05）; differentiation degree of tumor cells（X^2=6.32 ,P〈0.05）. （3） The expression of HIF-1α was correlated positively with that of Survivin.Conclusion So combined detection of expressions of HIF-1α and Survivin can be regarded as an important index to judge malignancy of the tumor and evaluate prognosis of cervical squamous cell carcinoma.