甘肃河西马铃薯根际生防木霉菌对接骨木镰刀菌的拮抗筛选及鉴定

Screening of biocontrol Trichoderma from potato rhizosphere of Western Gansu against Fusarium sambucinum and their identification

从甘肃省西部地区农牧交错区的马铃薯根际土壤中共分离到108株木霉菌株,通过平板对峙法筛选出10株对马铃薯干腐病主要病菌接骨木镰刀菌拮抗效果较好的木霉菌株,抗性系数均在0.826以上,其中天祝3株、永登4株、山丹3株。根据形态学特征、培养性状和ITS序列分析,将这10株生防木霉菌鉴定为哈茨木霉、长枝木霉、深绿木霉和粘绿木霉4个种。研究结果还表明,马铃薯根际土壤中的优势生防木霉菌种为哈茨木霉和长枝木霉,长枝木霉与深绿木霉对病原菌接骨木镰刀菌的拮抗作用最强。长枝木霉菌对接骨木镰刀菌有较好的拮抗效果,在国内系首次报道。

The 108 isolates of Trichoderma were acquired from potato rhizosphere in farming-pasturing zone of Western Gansu, 10 isolates of them had been acquired via the method of face to face on PDA to the pathogen Furuisam sambucinum causing potato dry rot. Through the identification based on morphology and analysis of ITS sequencing, these 10 isolates were identified as 4species including Trichoderma harzianum, T. longibrachiatum, T. atroviride and T. virens. T. harzianum and T. longibrachiatum are super Trichoderma species from potato rhizosphere in farming-pasturing zone of western Gansu, and these Trichoderma species had the best inhibition against F. sambucinum in this study. T. longibrachiatum showing superior inhibition effect against F. sambucinum is the first report in China.