三叶草愈伤组织诱导及分化的研究

A study on callus induction and redifferentiation of clover

以4个三叶草品种的胚轴、叶片和子叶为外植体,研究了不同激素浓度配比对愈伤组织诱导和分化的影响。结果表明,不同品种不同外植体愈伤诱导率差异较大,杂三叶下胚轴为较适宜的外植体,其愈伤组织诱导率和体细胞胚形成率最高可达96.7%和33.4%;改良SH+2,4-D 4.0 mg/L+KT 1 mg/L为最佳胚性愈伤组织诱导培养基,MSO培养基适宜三叶草胚状体的形成及植株再生。

The hypocotyls, leaf and cotyledon of four varieties of clover （Trifolum） were used to study the effect.of hormone concentrations on callus induction and redifferentiation. Different varieties and explants each had different induction requirements. The hypocotyl callus induction rate of Trifolium hybridum was high （96.7%）, while that of somatic embryogenesis was 33.4%. The best medium for embryogenic callus induction was modified SH＋2,4-D 4.0 mg/L＋KT 1 mg/L, while for embryoid redifferentiation and plant regeneration it was MSO.