荧光共振能量转移测定牛血清白蛋白的研究

Study on determination of bovine serum albumin by fluorescence resonance energy transfer

A new system of FRET was built in this paper.In the condition of λex/λem=400nm/560nm,0.1mol/L Tris buffer(pH=7.0),the effective energy transfer can occur between FITC(Fluorescein 5-isothiocyanate) and Rhodamine 6G(6G).The quenching of fluorescence of the system happened with the addition of BSA.According to that a new determination of BSA was developed.The quenching of this system liners with BSA from 10mg/L to 35mg/L,and the limit of determination is 9.5mg/L.The linear regression is F=0.0363×CBSA+0.7256 with the correlation coefficient,r=0.9970.

A new system of FRET was built in this paper. In the condition of λex/λem = 400nm/560nm, 0. 1 mol/L Tris buffer（ pH =7.0）, the effective energy transfer can occur between FITC （Fluoreseein 5-isothiocyanate） and Rhodamine 6G （6G）. The quenching of fluorescence of the system happened with the addition of BSA. According to that a new determination of BSA was developed. The quenching of this system liners with BSA from 10mg/L to 35mg/L, and the limit of determination is 9.5mg/L. The linear regression is F = 0.0363 × CBSA ＋ 0.7256 with the correlation coefficient, r = 0.9970.