HPLC-ESI-MS鉴定罗氏海盘车中的多种化合物及相关指纹图谱研究

Identification of active components in Asterias rollestoni Bell. by HPLC-ESI-MS and Study on Their HPLC Fingerprint

目的:建立高效液相色谱-电喷雾质谱(HPLC-ESI-MS)联用技术用于海洋中药罗氏海盘车有效成分快速鉴定及其指纹图谱研究的方法。方法:对罗氏海盘车药材进行超声波提取,采用HPLC-ESI-MS对罗氏海盘车粗提物中多种化合物进行分析,并在此基础上建立罗氏海盘车药材的HPLC特征指纹图谱。结果:结合文献,应用HPLC-ESI-MS鉴定出罗氏海盘车提取物中的11种化合物;在化合物鉴定的基础上,建立了罗氏海盘车药材的HPLC指纹图谱,发现不同批次罗氏海盘车的质量存在差异。结论:本方法可用于罗氏海盘车药材有效成分的快速鉴定及质量控制。

Objective： A new method based on high performance liquid chromatography- electrospray ionization mass spectrometry （HPLC-ESI-MS） was developed for the rapid identification of active compounds in Asterias rollestoni Bell and development of its fingerprints. Method： Samples were extracted by ultrasonic-assist extraction, and the extraction conditions were optimized. The developed HPLC-ESI-MS method was used to identification of the components in Asterias rollestoni Bell extract, and a chromatographic fingerprint based on HPLC analysis was established. Result： Eleven compounds in Asterias rollestoni Bell extract could be primary identified by ESI-MS on-line detection combined with literature review. The result of similarity evaluation for fingerprint indicated that the quality of different Asterias rollestoni Bell samples were not entirely consistent. Conclusion： This method has the advantages of simple operation, rapid measurement and it is a powerful tool for identification of active components in Asterias rollestoni Bell and its quality control.