摘要
以杂交兰的腋芽为材料,研究了杂交兰的组织培养和快速繁殖技术.结果表明:从诱导、增殖、分化到生根可以用同一种培养基,即MS+6-BA1mg/L+IBA0.5mg/L+活性炭5g/L+蔗糖30g/L+琼脂8g/L(pH5.8),IBA对杂交兰的增殖和分化影响较大.
Using axillary bud of Cymbidium hybridum× faberi,the tissue culture and plant regeneration was researched.The results show that MS＋6-BA 1.0 mg/L＋IBA 0.5 mg/L＋AC 5 g/L＋sucrose30 g/L＋agar 8 g/L（pH5.8） is the optimum medium for induction,increment,differentiation and induction of root;IBA affects deepey the increment and differentiation of Cymbidium hybridum×faberi.
出处
《河南科学》
2009年第4期419-421,共3页
Henan Science
基金
郑州市重点科技攻关项目(051SGDS17017)
关键词
杂交兰
圆球茎
分化
植株再生
Cymbidium hybridum×faberi
protocorm
differentiation
peant regeneration
