HPLC法测定银杏叶提取物中总黄酮醇苷的含量

HPLC Determination of Total Flavonoids in the Ginkgo Biloba Extract

目的：建立测定银杏叶提取物中总黄酮醇苷的含量测定方法.方法：用HPLC方法对测定银杏叶提取物中总黄酮醇苷进行含量测定研究.色谱柱采用Diamonsil C18（200×4.6 mm 5μm）,以甲醇-0.4%磷酸溶液（52∶48）为流动相,检测波长为360 nm;流速为1.0 mL/min.结果：槲皮素在0.174-0.870μg线性关系良好（r=0.999 8,n=5）,山柰素在0.117-0.585μg线性关系良好（r=0.999 8,n=5）,异鼠李素在0.040 8-0.204μg线性关系良好（r=0.999 9,n=5）;槲皮素、山柰素、异鼠李素的平均回收率（n=5）分别为99.96%、99.24%、100.46%.结论：本方法操作简便,重现性好,专属性强,可作银杏叶提取物中总黄酮醇苷的质量控制方法.

Objective：To establish an HPLC method for determination of flavones in the Ginkgo Biloba extract. Method： The chromatographic column was Diamonsil C18 （200 × 4.6 mm, 5 μm）, The mobile phase consisted of 0.5% phosphate buffer/methanol（48 ： 52）, the flow rate was 1.0 mL/min, UV detection wavelength was 360 nm. Results ： According to the selected chromatographic conditions, the linear ranges of quercetin, kaempferide and isorhamnetin were 0. 174 - 0. 870 μg, 0.117 - 0.585 μg and 0.040 8 - 0.204μg, respectively. The average recoveries of these three compounds were 99.96% ,99.24% and 100.46%, respectively. Conclusion：The method was simple, accurate, specific and would be used for the quality control of quercetin, kaemp ferol and isorhamnetin in the Ginkgo Biloba Extract.